Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural. Background Entamoeba histolytica, E. dispar and E. moshkovskii are the most frequent species described in human infection where E. This study investigated the presence of Entamoeba histolytica, Entamoeba dispar , and Entamoeba moshkovskii in stool samples from a patient population in.
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The Mother of All Pandemics — Length: Entamoeba moshkovskii cysts are morphologically indistinguishable from those of the disease-causing species E. Although sporadic cases of human infection with E.
No simple molecular detection tool is available for diagnosing E.
We used polymerase chain reaction PCR to detect E. In addition, we found that 23 The high association of E. The high prevalence found in this study suggests that humans may be a true host for this ameba. Entamoeba moshkovskiiconsidered to be primarily a free-living ameba, is indistinguishable in its cyst and trophozoite forms from E.
Although the early isolations of this species were from sewage, E. Human isolates of E. However, few studies have actually set out to identify such infections 7.
The structural resemblance of the apparently innocuous E. In the ehtamoeba setting, for example, an E. Most studies that have investigated the prevalence of E.
We report for the first time the application of tools to detect the species directly in stool and investigate the prevalence of E. Fecal specimens included in this study were from preschool children ages 2—5 years from Mirpur, an urban slum in Dhaka, Bangladesh. Of the 52 samples negative by stool PCR, 18 were eventually found positive for E. Only four mishkovskii the samples were from children with diarrhea.
Blacksburg, VA Entamoeba test designed to detect moshklvskii not differentiate E. In the initial PCR total vol. In the nested PCR, 1. All PCR products were separated in 1. Thermal cycler conditions for PCR were the following: From the sequence results, an Enatmoeba. Incubation led to growth of E. Hexokinase isoenzyme analysis was possible for 10 cultures; 4 of them showed the band pattern of Entamoeba histolytica5 showed E. The reference strain E. Of these, seven moshkovsmii positive for amebae by culture; one DNA sample extracted from these cultures was positive for E.
Seventeen of the 23 E. One of the four children with diarrhea was positive for E. The cause of his diarrhea remained undetermined. AFE. Products from all 23 positive stool samples and the Laredo strain showed the presence of this site Figure 2. To detect polymorphism among the E. The sizes of the PCR products from E. We did not observe a band in the to bp entxmoeba in any of the E.
Because 17 of 23 E. By this criterion, we found 18 of 23 samples were positive for Moshklvskii. The PCR products from one stool sample, E. EmR polymerase chain reaction products. Lane 1, Entamoeba moshkovskii Laredo; lane 2, E. The EmR primers amplified the expected bp fragment from E.
However, they successfully amplified moshkovkii of a possible 23 E. The most likely reason why these primers did not amplify the other 13 E. Although the PCR product size of the 10 positive samples was slightly different from that of Laredo, they were very similar in size to each other Figure 3. The DNA of the previously reported E. The main objectives of this study were to develop molecular tools to identify E. We were successful in developing a simple diagnostic technique: We chose to use nested PCR to detect E.
Our koshkovskii to produce a species-specific polymorphic marker was not completely successful.
Entamoeba moshkovskii infections in children, Bangladesh.
The EmR primers failed to amplify 13 of 23 E. Our study has some limitations. The subjects were children 2—5 years of age, so we entamoea not know whether these subjects are representive of all age groups.
All previous human isolates of E. Our attempts to perform riboprinting on these infections were unsuccessful, likely because of the size of the amplification target approximately 1.
Even if PCR had been successful, the presence of mixed infections moshkovsiki other eukaryotes would have prevented mosykovskii typing. This study has several important findings. None of the six children with E. The high prevalence of E. Previous attempts to identify human E. Epidemiologic studies of E. His current research focus includes genetic diversity in Entamoeba species. His interests include the diagnosis of Entamoeba species and parasite-related nutritional biochemistry.
Ali is funded by the Commonwealth Scholarship Commission. Table of Contents — Volume 9, Number 5—May Please use the form below to submit correspondence to the authors or contact them at the following address:.
Entamoeba moshkovskii infections in children, Bangladesh.
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Figures Figure 1 Figure 2 Figure 3. Tables Table 1 Table 2. Close Highlight and copy the desired format. Entamoeba moshkovskii Infections in Children in Bangladesh. Emerging Infectious Diseases9 5 Abstract Entamoeba moshkovskii cysts are morphologically indistinguishable from those of the disease-causing species E.
Stool Specimens Fecal specimens included in this study were from preschool children ages 2—5 years from Mirpur, an urban slum in Dhaka, Bangladesh. Antigen Detection Tests for E. Figure 2 Figure 2. Figure 3 Figure 3. Intraspecific variation and phylogenetic relationships in the genus Entamoeba as revealed by riboprinting.
Growth of a strain of Entamoeba histolytica at room temperature. Tex Rep Biol Med.
Cultivation of Entamoeba histolytica and Entamoeba histolytica -like strains at reduced temperature and behavior of the amebae in diluted media. Am J Trop Med Hyg. The Mosgkovskii strain and other Entamoeba histolytica —like amoebae are Entamoeba moshkovskii.
A case report of Entamoeba moshkovskii infection in a Bangladeshi child. Low temperature strains of Entamoeba histolytica. The laboratory diagnosis of human parasitic amoebae.
Entamoeba moshkovskii – Wikipedia
Methods for cultivation of luminal parasitic protists of clinical importance. Comparison of PCR, isoenzyme analysis, moshkovskui antigen detection for diagnosis of Entamoeba histolytica infection.
Direct amplification and differentiation of pathogenic and nonpathogenic Entamoeba histolytica DNA from stool specimens. Rapid diagnosis of Entamoeba infection by using Entamoeba and Entamoeba histolytica stool antigen detection kits. Comments Please use the form below to submit correspondence to the authors or contact them at the following address: Send To Authors Editors.
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